The Guns N' Roses rocker filed for divorce in Los Angeles Superior Court on Tuesday citing irreconcilable differences, ending their 13-year marriage, according to court records.
The 49-year-old British guitarist, real name Saul Hudson, is also seeking joint physical and legal custody of the couple's two sons, Cash, 10, and London, 12.
Slash wed Perla Hudson, formerly Perla Ferrar, on Oct. 15, 2001, and listed their date of separation as June 15, 2014.
It's the second divorce for the Rock and Roll Hall of Famer, who was previously wed to model Renee Suran. They split in 1997.
It's also the second time the Velvet Revolver co-founder tried to divorce this wife. He filed a petition back in 2010 but withdrew it two months later.
On Monday, TMZ reported that Perla, 39, flew to Hawaii with their sons to get Slash to work on his issues before the new year.
The rocker's management team did not respond to requests for comment.