Harrah's Entertainment officials said Williams died Wednesday in Las Vegas after a long illness.
"Claudine was a remarkable woman whose grace, tenacity, intellect, integrity, work ethic and community involvement propelled her to the chairmanship of Harrah's Las Vegas and to service on a wide range of business, charitable and community service organizations," Harrah's Chief Executive Gary Loveman said in a letter to company employees.
"Despite having only a ninth-grade education and competing in what was at the time an almost exclusively male-dominated industry, Claudine's achievements were remarkable."
In 1992, Williams was the first woman inducted into the American Gaming Assn.'s Gaming Hall of Fame.
Williams was born in 1921 in Mansfield, La., and moved as a teenager to Texas, where she met her husband, Shelby Williams. They bought the Silver Slipper casino on the Las Vegas Strip in the early 1960s and sold it several years later to billionaire Howard Hughes.
The couple bought property across from Caesars Palace and opened the riverboat-themed Holiday Casino adjacent to the Holiday Inn. After Shelby Williams died in 1977, Claudine Williams emerged as president and general manager of the Holiday.
She sold 40% of the casino in 1979 to Holiday Inns Inc., then the parent company of Harrah's Entertainment, which -- by then spun off -- bought her remaining interest in 1983.
Williams retained the title of chairman of the renamed Harrah's Las Vegas, the company's first casino in town.
Harrah's, then based in Reno, and now in Las Vegas and privately held, owns or operates more than 50 casinos, including eight in Las Vegas. Its revenue in 2008 was $9.4 billion.
Williams served on more than two dozen boards and commissions over the years, including the Las Vegas Convention and Visitors Authority, and contributed millions of dollars to charities and to the University of Nevada, Las Vegas. A new campus dormitory was named for her in 1989.
Williams is survived by a son, Michael Shelby Williams of New York; a stepson, Scott Noe; and a stepdaughter, Susan Noe of Houston.