Despite a surge in revenue,
The San Francisco company reported revenue of $250 million, up 119% year over year, for the three months ended March 31. It had a net loss of $132 million, or 23 cents, compared with a net loss of $27 million in the same quarter a year earlier.
The microblogging platform said its net loss included $126 million of stock-based compensation expense.
Excluding one-time events, Twitter's earnings per share were flat.
Analysts were expecting Twitter to report a loss of 3 cents a share, on revenue of $241.46 million, according to a survey by Factset.
Twitter reported its earnings after the markets closed. During regular trading, shares rose $1.89, or 4.64%, to $42.62.
In after-hours trading, shares were down $4.14, or 9.71%, to $38.48 at 1:40 p.m. PDT.
Twitter again struggled with user growth, a problem area during its last earnings report.
Twitter had 255 million monthly active users as of March 31, an increase of 25% year over year; analysts had expected 257 million. That also represented a slowdown in growth: Twitter had reported a 30% year-over-year increase in monthly active users during its last earnings report three months ago.
Mobile monthly active users reached 198 million in the first quarter, which represented 78% of the total.
Timeline views reached 157 billion for the first quarter, an increase of 15% year over year.
"We had a very strong first quarter. Revenue growth accelerated on a year-over-year basis fueled by increased engagement and user growth," Chief Executive Dick Costolo said. "We also continue to rapidly increase our reach and scale."
Advertising revenue totaled $226 million, up 125%. Mobile advertising revenue was about 80% of total advertising revenue.
Advertising revenue per thousand timeline views reached $1.44 in the first quarter of 2014, an increase of 96% year over year.