The pop superstars are expected to be among the big surprise moments during next week's ceremony, according to two sources with knowledge of the plans.
Late last year Beyonce pulled off one of the best-kept secrets in recent music history when she stealthily released her self-titled album without as much as a hint.
Madonna, however, is expected to pop up and lend guest vocals during a performance from one of the night's nominees, an additional source confirmed.
Longtime Grammy Awards show producer Ken Ehrlich said he "can't confirm" an appearance from Beyonce during an interview with Pop & Hiss to preview the show. Ehrlich didn't confirm or deny Madonna's involvement.
"Let's put it this way … we have a really great history that goes back to the early days of Destiny's Child," Ehrlich added. "I love working with [Beyonce]. She's incredible. She is so into what it is that she does and making sure that everybody who sees her walks away saying, 'wow.' "
"Should she be on the show this year?" he continued. "I would think that the potential is there for that kind of moment."
Other Grammy performers include
Reps for Madonna and Beyonce had yet to return requests for comment.
The Grammy telecast airs at 8 p.m. Eastern and Pacific time Jan. 26.