Culliver was arrested on suspicion of hit-and-run, reckless driving with injury and weapons possession, San Jose police said.
Culliver, 25, was driving a white Ford Mustang when police said he struck a bicyclist and attempted to flee the scene, NBC Bay Area reported. The bicyclist was crossing at the intersection when he was struck.
A witness followed Culliver and attempted to block the
But when Culliver was cornered by the witness, officers said the athlete threatened him with brass knuckles before getting back into his car and driving away, hitting the witness' car in the process.
When police arrived, they took Culliver into custody without incident, the station reported.
In a statement, the San Francisco 49ers told NBC Bay Area that the team was "aware of the recent matter involving Chris Culliver."
"We will remain in contact with Chris and the local authorities as we continue to gather the facts regarding this situation. As this is an ongoing legal matter, we will reserve further comment at this time."
Culliver previously made headlines for anti-gay remarks he made made during a Super Bowl media day interview two years ago.