Lizzy failed to heed Mirren's command during a scene set in Balmoral, the British monarch's retreat in Scotland. The dog was supposed to run across stage without a leash when called by the actress, but failed to do so during several previews.
The Telegraph reported that Lizzy, who is 7 years old, failed to obey the command on 16 consecutive performances. As a result, Lizzy was sacked and replaced with another dog, named Coco.
Lizzy is now back home with her owner, according to the report.
"The Audience," by
Mirren previously played the British monarch in the 2006 movie "The Queen," which was also written by Morgan.
The new play follows the queen during her meetings with various prime ministers, including Winston Churchill,
The queen's official website states that she owns two Corgis: Holly and Willow. The queen also has two Dorgis (a cross-breed of Dachshund and Corgi): Candy and Vulcan. One of her corgis, Monty, died at Balmoral in September at the age of 13.