Icahn said he decided it was time to cash out, after the rapid appreciation of the company's stock, which rose to $323 a share, from his initial investment of $58 a share.
"As a veteran of seven bear markets, I have learned that when you are lucky and/or smart enough to have made a total return of 457% in only 14 months, it is time to take some chips off the table," Icahn said in a statement.
After the sale, Icahn retains a stake of 4.5%, with nearly 2.7 million shares.
Son Brett Icahn and a manager at Icahn Enterprises, David Schechter, issued a statement saying they felt the company remains "significantly undervalued."
"As a subscription service priced at only $7.99 per month, we believe Netflix is one of the great consumer bargains of our time," Schechter and Brett Icahn wrote. "We find it difficult to understand why a household would not subscribe to the service."
The younger Icahn and Schechter wrote that they share Netflix Chief Executive Reed Hastings' vision that the company could eventually reach 60 million to 90 million domestic subscribers. They estimate that with these subscriber additions -- and the "reasonable" prospect of an eventual price hike to $10 a month over the next five years -- the company would raise its domestic streaming revenue by $4.3 billion a year.
While the domestic growth story is compelling, they wrote, the international opportunity is even larger.
"As these international markets mature, we expect the aggregate international operating profits will actually exceed the domestic," Brett Icahn and Schechter wrote.
Netflix stock closed at $322.47 Tuesday, down 9% for the day. The stock had rallied in after-hours trading Monday, after the company reported earnings of $32 million, or 52 cents a share, for the three months ended Sept. 30. That's four times its earnings from the same quarter last year. Revenue reached $1.1 billion, up 22% from the same period in 2012.
Some analysts raised questions about the service's "low-quality promotions" in Latin America, and how it could affect profitability. Others noted the paltry $7 million in free cash.
Chief Financial Officer David Wells told investors the company is changing how it validates subscribers for its free trials.
"You've got a lot of people in a free trial state that aren't going to become paid subscribers," Well said Monday, during a
[For the record, 5:50 p.m. PDT Oct. 22: An earlier version of this post said Netflix's domestic streaming revenue was estimated to reach $4.3 billion a year. It was estimated to grow by that amount.]