Riley filed to divorce Musk on Monday in Los Angeles Superior Court. Musk filed for divorce on New Year's Eve in 2014 but withdrew the petition seven months later.
The divorce is amicable and the pair agreed that Riley would file the petition to end their latest marriage after roughly 2 ½ years. According to the statement, the Musk and Riley have been living separately for the past six months and plan to remain friends.
Musk co-founded Paypal and has gone on to help start the electric car company
The pair first married in 2010 and divorced in 2012. They re-married 18 months later.
Riley sat in the front row at September's unveiling of Tesla's Model X sport utility vehicle. Musk is scheduled to unveil the newest version of Tesla's electric car, the lower-cost Model 3, at an event later this month.
Musk and Riley do not have any children together, although he has five sons from a previous marriage.