Donovan scored two goals and added an assist in the Galaxy's 4-1 win over the Philadelphia Union at StubHub Center on Sunday.
The goals enabled Donovan to claim sole possession of the MLS career scoring record with 136. He had been tied with Jeff Cunningham at 134.
Donovan's latest scores came three days after Coach Juergen Klinsmann set off widespread debate by cutting the 32-year-old Donovan from the U.S. World Cup team.
Donovan, a three-time World Cup veteran, is U.S. Soccer's all-time leader in goals and assists, and Donovan said he was sad and disappointed by Klinsmann's decision.
The Galaxy next plays the Fire in Chicago on Sunday.