Seattle designated hitter
Segura injured his right ankle sliding into second base in the fourth inning. Cruz was hit on the top of his left hand by a pitch from Colorado starter Kyle Freeland in the third inning and was pulled for a pinch hitter in the fifth.
The team said both players were being examined.
"Hopeful that guys are not out for an extended period of time, we certainly need them, but injuries happen and we've dealt with it all year and we'll deal with this if we lose the guys," Seattle manager Scott Servais said. "But I am hopeful it's only going to be a day-to-day thing with both of those guys. We'll just have to wait and see."
Both Cruz and Segura were getting additional tests and not in the clubhouse after Seattle's four-game winning streak was stopped. Servais called Segura's injury a twisted ankle, although it looked like it could be the more serious of the two.