Celebrating the pending nuptials with friends and family in southern Italy since Tuesday, the actor-singer and actress finally tied the knot in a Friday ceremony.
"It's great to be married, the ceremony was beautiful and it was so special to be surrounded by our family and friends," the pair said in a joint statement to People.
Guests included funnyman Andy Samberg, Biel's "7th Heaven" costar Beverly Mitchell and music manager Johnny Wright.
On Wednesday, the group enjoyed cocktails and a fireworks display at beach club Cala Masciola ahead of the big day.
Biel and Timberlake have been an item since 2007, splitting briefly in March 2011. Their reconciliation led to a December proposal in which Timberlake offered her a custom engagement ring.